The article aims to analyse the effects of the start-up subsidy programme for unemployed in the Czech Republic, which is provided by the Ministry of Labour and Social Affairs. We explored to what extent participants in the programme in 2014 returned to unemployment three years after the end of the programme, i.e., 2014–2017. Methodologically, we conducted counterfactual impact analysis, where we matched participants in the programme with those who were not supported by any measures of active labour market policy. The results show that participants in the programme return to unemployment to a lesser extent. In total, they spent fewer days in unemployment during the follow-up period, and they returned to unemployment fewer times when compared with non-participants. Overall, 91.3% of participants never returned to unemployment during the analysed period. These results can be interpreted as a positive outcome of the programme. The article also offers implications for targeting the programme and for future research.